Gh Serum Testing Schedule

[madmoe]

This is good info. We are doing same testing and results are much the same-Thanks-OD

Bro, this is what is all about. It is about helping each other, comparing notes, agreeing to disagree sometimes on hot topics, etc. Like I said in a previous post, we are in ALL in this shit together, most of us will never step onto a BB stage but most of us do end up dealing with pretty much the same sources for this gear.

Whether we like to hear it or not, very very few of us know whose these sources really are, even fewer know how the GH is produced in China. So from our end-user experience over here all we can do is test this stuff to the best of our ability and go from there. What got to me lately is how little do we really know about the GH produced outside of some bro saying "I got lots of CTS so therefore it must be good" or "I'm leaning out like crazy therefore it must be the GH".

My wife asked me a question a couple of weeks ago that got me thinking:

" If one of your bros said that one of his sources was home-brewing some type of gasoline additive in his backyard would you put it in your $65K car?"

My answer was "HELL NO!!" Well, most of you can guess what her retort was...

"So why the hell are you sticking this stuff in your body from some guy in China that you have never even met nor has anyone you know really tested?"

I felt like a fucking 6 year old...

 

[JimmyjackDuke]

Bro, this is what is all about. It is about helping each other, comparing notes, agreeing to disagree sometimes on hot topics, etc. Like I said in a previous post, we are in ALL in this shit together, most of us will never step onto a BB stage but most of us do end up dealing with pretty much the same sources for this gear.

Whether we like to hear it or not, very very few of us know whose these sources really are, even fewer know how the GH is produced in China. So from our end-user experience over here all we can do is test this stuff to the best of our ability and go from there. What got to me lately is how little do we really know about the GH produced outside of some bro saying "I got lots of CTS so therefore it must be good" or "I'm leaning out like crazy therefore it must be the GH".

My wife asked me a question a couple of weeks ago that got me thinking:

" If one of your bros said that one of his sources was home-brewing some type of gasoline additive in his backyard would you put it in your $65K car?"

My answer was "HELL NO!!" Well, most of you can guess what her retort was...

"So why the hell are you sticking this stuff in your body from some guy in China that you have never even met nor has anyone you know really tested?"

I felt like a fucking 6 year old...

I like how your wife thinks ... that is some funny shit.

I would have answered the SAME way.....

She is right though. That plays in the back of my mind all the time.
F-N chinese have a hard time making drywall, and kids toys that are non-toxic!

That is why I have been so interested in pharm grade lately.

I am pretty sure there is GH in what we get from china, it is just all the other shit that may have fallen in that scares me.

 

[Micro]

Yeah, I just want to be clear about one thing:

This is how it affects MY BODY. I cannot guarantee it will be the same results on someone else, but this is good. price per IU/potency ratio compared with PHARMA is HUGE.

Rips are next. I will have the results tomorrow.

I wish I could get my results that quick. It's been a week for me and I still don't have my lab results back yet.

 

[roidrunner]

Bro, this is what is all about. It is about helping each other, comparing notes, agreeing to disagree sometimes on hot topics, etc. Like I said in a previous post, we are in ALL in this shit together, most of us will never step onto a BB stage but most of us do end up dealing with pretty much the same sources for this gear.

Whether we like to hear it or not, very very few of us know whose these sources really are, even fewer know how the GH is produced in China. So from our end-user experience over here all we can do is test this stuff to the best of our ability and go from there. What got to me lately is how little do we really know about the GH produced outside of some bro saying "I got lots of CTS so therefore it must be good" or "I'm leaning out like crazy therefore it must be the GH".

My wife asked me a question a couple of weeks ago that got me thinking:

" If one of your bros said that one of his sources was home-brewing some type of gasoline additive in his backyard would you put it in your $65K car?"

My answer was "HELL NO!!" Well, most of you can guess what her retort was...

"So why the hell are you sticking this stuff in your body from some guy in China that you have never even met nor has anyone you know really tested?"

I felt like a fucking 6 year old...

Because there's a difference between paying $4/gallon and .50cents/gallon for gas as oppose to $150/vial vs $150/kit. All of us would be using pharm grade if we had unlimited access to it and the funds for it. Mutation begins at a vial a day. Some, and only some, can afford the $450/month for Chinese gh. How many can afford $4,500/month for pharm grade?

This is an awesome thread and what boards like these are for. You're doing God's work madmoe. Thank you so much for going through all the time, trouble and expense in sharing this info. You are helping a lot of people. People who are just goddamn tired of getting raped over and over by scammers and crooks.

 

[roidrunner]

Didn't expect that. So does that mean Novos are as good as thanks and pharm grade? And having different hgh brands built up in your system from days prior won't effect the results?

This is a very good point. I believe the igf builds up over time but I don't know squat about gh serum testing. Say, after testing the Rips he goes back and test another vial of Thanks from the same batch and they come back even higher. Couldn't we suspect or conclude that serum levels have built up from previous hgh administration?

 

[nilla]

Good points.

 

[roidrunner]

I like how your wife thinks ... that is some funny shit.

I would have answered the SAME way.....

She is right though. That plays in the back of my mind all the time.
F-N chinese have a hard time making drywall, and kids toys that are non-toxic!

That is why I have been so interested in pharm grade lately.

I am pretty sure there is GH in what we get from china, it is just all the other shit that may have fallen in that scares me.

Make no mistake about it. The Chinks can make very good gh if they want to. They take sports, especially international sports; very, very seriously. Like all Communist countries they consider it a testament to their superior political system and race. Your kid shows some talent in gymnastics they will take her aways from her family and house her in a gymnastic center and squeeze every last bit out her and give her anything and everything to make that happen.

 

[fourthforce]

This is a very good point. I believe the igf builds up over time but I don't know squat about gh serum testing. Say, after testing the Rips he goes back and test another vial of Thanks from the same batch and they come back even higher. Couldn't we suspect or conclude that serum levels have built up from previous hgh administration?

the gh serum level goes back down after 12 hours or so i believe

 

[Micro]

This is a very good point. I believe the igf builds up over time but I don't know squat about gh serum testing. Say, after testing the Rips he goes back and test another vial of Thanks from the same batch and they come back even higher. Couldn't we suspect or conclude that serum levels have built up from previous hgh administration?

I had the highest IGF levels when I stopped GH, compared to when I was on. I can't remember off hand but I think I got tested about 1 month or 2 after I stopped GH and it was higher compared to when I was on GH.

 

[Micro]

the gh serum level goes back down after 12 hours or so i believe

If that's the cast then that would be good for this kind of testing.

 

[bojangles2112]

testing

I am going Thrusday for bloodwork. I am currently using redtops. Will post results IGF! and serum gh.

 

[AvEnged]

Only a suggestion. Would it be possible if you took the test on the same schedule you would, but with no gh shot prior. Then we could see if your levels do actually drop down within in normal range before you test a new brand each time....

 

[ordawg1]

Bro, this is what is all about. It is about helping each other, comparing notes, agreeing to disagree sometimes on hot topics, etc. Like I said in a previous post, we are in ALL in this shit together, most of us will never step onto a BB stage but most of us do end up dealing with pretty much the same sources for this gear.

Whether we like to hear it or not, very very few of us know whose these sources really are, even fewer know how the GH is produced in China. So from our end-user experience over here all we can do is test this stuff to the best of our ability and go from there. What got to me lately is how little do we really know about the GH produced outside of some bro saying "I got lots of CTS so therefore it must be good" or "I'm leaning out like crazy therefore it must be the GH".

My wife asked me a question a couple of weeks ago that got me thinking:

" If one of your bros said that one of his sources was home-brewing some type of gasoline additive in his backyard would you put it in your $65K car?"

My answer was "HELL NO!!" Well, most of you can guess what her retort was...

"So why the hell are you sticking this stuff in your body from some guy in China that you have never even met nor has anyone you know really tested?"

I felt like a fucking 6 year old...

Great points from the bride for sure.I alawys try to get guys to do a male panel when using test too.Know your baselines- learn your esters and what your level should be X amount of days after inject.This also keeps the sources honest. Great work my friend-Thanks for sharing-sent you a PM-Thanks-OD

 

[madmoe]

Regarding HGH Serum Levels after a 24 hour period

Please see in red

http://edrv.endojournals.org/content/28/6/603.full#ref-230

The Growth Hormone/Insulin-Like Growth Factor-I Axis in Exercise and Sport

(EXERPT)


B. Tests to detect GH doping by athletes

It is important to detect abuse of GH in the interest of fair competition and also because, as illustrated by the pathophysiological model of acromegaly, long-standing elevation of GH and IGF-I is detrimental to health (215). However, a number of factors complicate GH detection. Exogenous r-hGH and endogenous GH have identical amino acid sequences, making chemical distinction impossible. GH is secreted in a pulsatile manner; is under the influence of stress, exercise, sleep, and food intake (89); and has a very short half-life in the circulation (216), resulting in serum concentrations that vary widely throughout the day and frequently overlap with measurements obtained after exogenous administration of GH. The concentration of GH (like other proteins) in urine varies markedly with exercise and has been previously demonstrated to be insensitive as a marker of either GH administration or acromegaly (217, 218). It is very likely, therefore, that any useful test for GH abuse will involve blood sampling, which represents a major change from the long-established antidoping methods that are based on postcompetition urine tests.

The second potentially useful approach to developing a test for GH abuse involves simultaneous RIA of the natural isoforms and fragments of GH, the two most commonly occurring being 22-kDa GH and 20-kDa GH. Exogenous administration of supraphysiological doses of r-hGH, which consists exclusively of 22-kDa GH, suppresses endogenous GH secretion and therefore increases the ratio in plasma of 22-kDa to 20-kDa GH (228). Preliminary studies evaluating this approach have shown promise (229, 230), although in view of the short half-life of GH in the circulation, this test is only likely to be effective if sampling is carried out within 24 h of the last GH injection. Furthermore, there is a more marked increment in circulating 22-kDa GH compared with 20-kDa GH levels in response to exercise (229), and therefore the sensitivity of the test could be reduced in the postcompetition setting. Pituitary-derived GH consisting of multiple isoforms and fragments is still in circulation, and its use will not be detected. Finally, it must be considered that recombinant 20-kDa GH has also been synthesized, and therefore appropriate combinations of 20- and 22-kDa r-hGH could potentially confound this test. Of note, this test was introduced in the Olympic Games in 2004 and the Winter Olympics in 2006, but no positive tests were recorded.

...Detection of exogenous rhGH administration by measuring molecular isoforms of GH was therefore reliable, 3 h (but not 24 h or more) after the last dose. ....

 

[madmoe]

A robust test for growth hormone doping – present status and
future prospects
Anne E. Nelson, Ken K. Ho
Pituitary Research Unit, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia

http://www.nature.com/aja/journal/v10/n3/pdf/aja2008254a.pdf

Following administration of recombinant 22K GH, there is an increase in the relative abundance of 22K GH\ compared to the other forms of GH, and the Rec : Pit
ratio is increased. Good separation of the Rec : Pit ratio
for GH-treated versus control samples (Rec : Pit ratio
GH: 1.43 ± 0.21 vs. control 0.50 ± 0.12, mean ± SD) has
been reported [38]. The window of opportunity for detection is relatively short, possibly up to 24–36 h after
the last GH injection [30]. To meet the requirement of
WADA for a confi r matory t est for a ny i mmunologi cal
assay using a different antibody that recognizes a different epitope of the peptide or protein being assayed, the
Strasburger group has further established another pair
of Rec and Pit assays using different specific MAbs [39].
An alternate isoform-based method for detection of
exogenous GH has been developed using measurement
of the specific 20K GH isoform, together with measurement of 22K GH. Specific MAbs to 20K GH have been
raised that do not cross-react with 22K GH and a specific sandwich ELISA established for the measurement
of 20K GH in human serum [40, 41]. Co-secretion of
20K GH with 22K GH, with peaks of secretion coinciding during the day, has been demonstrated, indicating
that under normal physiological conditions, the circulating concentration of 20K is in a constant proportion to
22K GH [26, 41] (Figure 2). Administration of exogenous GH, however, results in rapid reduction of 20K
GH concentration, due to negative feedback regulation
on pituitary secretion of 20K GH (Figure 2). Following
injection of exogenous 22K GH, the increase in circulating 22K and the reduction in 20K GH result in a rapid
change in the ratio of 22K to 20 K GH for up to 24 h [26].
Current studies from our group on the changes in the
ratio following daily injections of 2 mg GH for 8 weeks
also suggest that the window of opportunity for detection might be within 24 h of injection (unpublished results).

 

[Premium]

This is one of the best threads i've seen in ages.

Thanks for putting this to rest and going through the trouble to help all of us out.


-Premium

 

[madmoe]

Only a suggestion. Would it be possible if you took the test on the same schedule you would, but with no gh shot prior. Then we could see if your levels do actually drop down within in normal range before you test a new brand each time....

Thinking about it brother... every study I read is saying the same thing about 24 hours GH half life... but why not do it and put it to rest? Maybe I will stop by tomorrow and get it done. PrivateMD should give me a better discount!

Thinking about going right before Labcorp close tomorrow, since that will be around 33+ hours after my last injection. Wednesday I will be doing Elis instead of Friday(going out of town with the family for the weekend).

I may be adding some other GH brands to the testing protocol. I got a few exciting items coming in, but I will not be able to disclose them until they are in route.

But as a preview: I will be testing REAL JINS and GENERIC JINS. I wonder which one will test higher? lol.

 

[roidrunner]

Thinking about it brother... every study I read is saying the same thing about 24 hours GH half life... but why not do it and put it to rest? Maybe I will stop by tomorrow and get it done. PrivateMD should give me a better discount!

Thinking about going right before Labcorp close tomorrow, since that will be around 33+ hours after my last injection. Wednesday I will be doing Elis instead of Friday(going out of town with the family for the weekend).

I may be adding some other GH brands to the testing protocol. I got a few exciting items coming in, but I will not be able to disclose them until they are in route.

But as a preview: I will be testing REAL JINS and GENERIC JINS. I wonder which one will test higher? lol.

I can't thank you enough for starting up this thread. I never heard about any of this before. This should change this whole gh scamming game. You're a freakin' saint in this whole sordid cult of ours. BTW, what does the lab say about you coming in there eod to get tested? Do they even give a crap?

 

[madmoe]

I can't thank you enough for starting up this thread. I never heard about any of this before. This should change this whole gh scamming game. You're a freakin' saint in this whole sordid cult of ours. BTW, what does the lab say about you coming in there eod to get tested? Do they even give a crap?

Not really. She looks at me weird, but I'm a paying customer so who gives a crap? It is not like I'm doing a drug screen EOD.

 

[Spicyer]

I've always done my hgh serum tests in a fasting state, the paperwork from MD Labs says FASTING on it. I know when testing igf levels, fasting vs. non has a huge difference, do you think results could be skewed due to nonfasting?