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  #51  
Old 02-27-2012, 06:32 PM
bojangles2112 bojangles2112 is offline
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Default testing

I am going Thrusday for bloodwork. I am currently using redtops. Will post results IGF! and serum gh.
  #52  
Old 02-27-2012, 06:52 PM
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Only a suggestion. Would it be possible if you took the test on the same schedule you would, but with no gh shot prior. Then we could see if your levels do actually drop down within in normal range before you test a new brand each time....
  #53  
Old 02-27-2012, 07:06 PM
ordawg1 ordawg1 is offline
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Bro, this is what is all about. It is about helping each other, comparing notes, agreeing to disagree sometimes on hot topics, etc. Like I said in a previous post, we are in ALL in this shit together, most of us will never step onto a BB stage but most of us do end up dealing with pretty much the same sources for this gear.

Whether we like to hear it or not, very very few of us know whose these sources really are, even fewer know how the GH is produced in China. So from our end-user experience over here all we can do is test this stuff to the best of our ability and go from there. What got to me lately is how little do we really know about the GH produced outside of some bro saying "I got lots of CTS so therefore it must be good" or "I'm leaning out like crazy therefore it must be the GH".

My wife asked me a question a couple of weeks ago that got me thinking:

" If one of your bros said that one of his sources was home-brewing some type of gasoline additive in his backyard would you put it in your $65K car?"

My answer was "HELL NO!!" Well, most of you can guess what her retort was...

"So why the hell are you sticking this stuff in your body from some guy in China that you have never even met nor has anyone you know really tested?"

I felt like a fucking 6 year old...
Great points from the bride for sure.I alawys try to get guys to do a male panel when using test too.Know your baselines- learn your esters and what your level should be X amount of days after inject.This also keeps the sources honest. Great work my friend-Thanks for sharing-sent you a PM-Thanks-OD
  #54  
Old 02-27-2012, 10:03 PM
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Default Regarding HGH Serum Levels after a 24 hour period

Please see in red

http://edrv.endojournals.org/content...3.full#ref-230

The Growth Hormone/Insulin-Like Growth Factor-I Axis in Exercise and Sport

(EXERPT)


B. Tests to detect GH doping by athletes

It is important to detect abuse of GH in the interest of fair competition and also because, as illustrated by the pathophysiological model of acromegaly, long-standing elevation of GH and IGF-I is detrimental to health (215). However, a number of factors complicate GH detection. Exogenous r-hGH and endogenous GH have identical amino acid sequences, making chemical distinction impossible. GH is secreted in a pulsatile manner; is under the influence of stress, exercise, sleep, and food intake (89); and has a very short half-life in the circulation (216), resulting in serum concentrations that vary widely throughout the day and frequently overlap with measurements obtained after exogenous administration of GH. The concentration of GH (like other proteins) in urine varies markedly with exercise and has been previously demonstrated to be insensitive as a marker of either GH administration or acromegaly (217, 218). It is very likely, therefore, that any useful test for GH abuse will involve blood sampling, which represents a major change from the long-established antidoping methods that are based on postcompetition urine tests.

The second potentially useful approach to developing a test for GH abuse involves simultaneous RIA of the natural isoforms and fragments of GH, the two most commonly occurring being 22-kDa GH and 20-kDa GH. Exogenous administration of supraphysiological doses of r-hGH, which consists exclusively of 22-kDa GH, suppresses endogenous GH secretion and therefore increases the ratio in plasma of 22-kDa to 20-kDa GH (228). Preliminary studies evaluating this approach have shown promise (229, 230), although in view of the short half-life of GH in the circulation, this test is only likely to be effective if sampling is carried out within 24 h of the last GH injection. Furthermore, there is a more marked increment in circulating 22-kDa GH compared with 20-kDa GH levels in response to exercise (229), and therefore the sensitivity of the test could be reduced in the postcompetition setting. Pituitary-derived GH consisting of multiple isoforms and fragments is still in circulation, and its use will not be detected. Finally, it must be considered that recombinant 20-kDa GH has also been synthesized, and therefore appropriate combinations of 20- and 22-kDa r-hGH could potentially confound this test. Of note, this test was introduced in the Olympic Games in 2004 and the Winter Olympics in 2006, but no positive tests were recorded.

...Detection of exogenous rhGH administration by measuring molecular isoforms of GH was therefore reliable, 3 h (but not 24 h or more) after the last dose. ....
  #55  
Old 02-27-2012, 10:11 PM
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A robust test for growth hormone doping present status and
future prospects
Anne E. Nelson, Ken K. Ho
Pituitary Research Unit, Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia

http://www.nature.com/aja/journal/v1...ja2008254a.pdf

Following administration of recombinant 22K GH, there is an increase in the relative abundance of 22K GH\ compared to the other forms of GH, and the Rec : Pit
ratio is increased. Good separation of the Rec : Pit ratio
for GH-treated versus control samples (Rec : Pit ratio
GH: 1.43 0.21 vs. control 0.50 0.12, mean SD) has
been reported [38]. The window of opportunity for detection is relatively short, possibly up to 2436 h after
the last GH injection [30].
To meet the requirement of
WADA for a confi r matory t est for a ny i mmunologi cal
assay using a different antibody that recognizes a different epitope of the peptide or protein being assayed, the
Strasburger group has further established another pair
of Rec and Pit assays using different specific MAbs [39].
An alternate isoform-based method for detection of
exogenous GH has been developed using measurement
of the specific 20K GH isoform, together with measurement of 22K GH. Specific MAbs to 20K GH have been
raised that do not cross-react with 22K GH and a specific sandwich ELISA established for the measurement
of 20K GH in human serum [40, 41]. Co-secretion of
20K GH with 22K GH, with peaks of secretion coinciding during the day, has been demonstrated, indicating
that under normal physiological conditions, the circulating concentration of 20K is in a constant proportion to
22K GH [26, 41] (Figure 2). Administration of exogenous GH, however, results in rapid reduction of 20K
GH concentration, due to negative feedback regulation
on pituitary secretion of 20K GH (Figure 2). Following
injection of exogenous 22K GH, the increase in circulating 22K and the reduction in 20K GH result in a rapid
change in the ratio of 22K to 20 K GH for up to 24 h [26].
Current studies from our group on the changes in the
ratio following daily injections of 2 mg GH for 8 weeks
also suggest that the window of opportunity for detection might be within 24 h of injection (unpublished results).
  #56  
Old 02-27-2012, 10:15 PM
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This is one of the best threads i've seen in ages.

Thanks for putting this to rest and going through the trouble to help all of us out.


-Premium
  #57  
Old 02-27-2012, 10:21 PM
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Quote:
Originally Posted by AvEnged View Post
Only a suggestion. Would it be possible if you took the test on the same schedule you would, but with no gh shot prior. Then we could see if your levels do actually drop down within in normal range before you test a new brand each time....
Thinking about it brother... every study I read is saying the same thing about 24 hours GH half life... but why not do it and put it to rest? Maybe I will stop by tomorrow and get it done. PrivateMD should give me a better discount!

Thinking about going right before Labcorp close tomorrow, since that will be around 33+ hours after my last injection. Wednesday I will be doing Elis instead of Friday(going out of town with the family for the weekend).

I may be adding some other GH brands to the testing protocol. I got a few exciting items coming in, but I will not be able to disclose them until they are in route.

But as a preview: I will be testing REAL JINS and GENERIC JINS. I wonder which one will test higher? lol.

Last edited by madmoe : 02-27-2012 at 10:26 PM.
  #58  
Old 02-27-2012, 11:52 PM
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Originally Posted by madmoe View Post
Thinking about it brother... every study I read is saying the same thing about 24 hours GH half life... but why not do it and put it to rest? Maybe I will stop by tomorrow and get it done. PrivateMD should give me a better discount!

Thinking about going right before Labcorp close tomorrow, since that will be around 33+ hours after my last injection. Wednesday I will be doing Elis instead of Friday(going out of town with the family for the weekend).

I may be adding some other GH brands to the testing protocol. I got a few exciting items coming in, but I will not be able to disclose them until they are in route.

But as a preview: I will be testing REAL JINS and GENERIC JINS. I wonder which one will test higher? lol.
I can't thank you enough for starting up this thread. I never heard about any of this before. This should change this whole gh scamming game. You're a freakin' saint in this whole sordid cult of ours. BTW, what does the lab say about you coming in there eod to get tested? Do they even give a crap?
  #59  
Old 02-27-2012, 11:56 PM
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Originally Posted by roidrunner View Post
I can't thank you enough for starting up this thread. I never heard about any of this before. This should change this whole gh scamming game. You're a freakin' saint in this whole sordid cult of ours. BTW, what does the lab say about you coming in there eod to get tested? Do they even give a crap?
Not really. She looks at me weird, but I'm a paying customer so who gives a crap? It is not like I'm doing a drug screen EOD.
  #60  
Old 02-28-2012, 12:14 AM
Spicyer Spicyer is offline
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I've always done my hgh serum tests in a fasting state, the paperwork from MD Labs says FASTING on it. I know when testing igf levels, fasting vs. non has a huge difference, do you think results could be skewed due to nonfasting?
  #61  
Old 02-28-2012, 12:21 AM
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I've always done my hgh serum tests in a fasting state, the paperwork from MD Labs says FASTING on it. I know when testing igf levels, fasting vs. non has a huge difference, do you think results could be skewed due to nonfasting?
Fasting is required when testing endogenous gh release and production. Fasting is only required during endogenous testing because it affects internal production and release of GH (natural production). By injection large amounts of GH exogenously (10 IU) we are testing & hoping to obtain usually 20-50 plus times the endogenous production. Therefore food intake cannot have measurable effect on taking exogenous GH prior to testing.

Again this is just my bro opinion.
  #62  
Old 02-28-2012, 03:53 AM
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Not really. She looks at me weird, but I'm a paying customer so who gives a crap? It is not like I'm doing a drug screen EOD.
Yeah, I use to get the same look when I would rent vhs porn tapes eod before the internet took over the industry. But I was a paying customer. But I have to admit things did get a little tense and creepy when I went through my granny porn phase.
  #63  
Old 02-28-2012, 07:38 AM
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This is great information, you are doing a great service. I had good results on Hyges, but we know what happened to them. I finally was able to get on pharm grade HGH. It is crazy expensive, but the results are very good.
  #64  
Old 02-28-2012, 11:23 AM
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Thumbs up Riptropin Results from Labcorp

LABTEST DATE--------------------- HGH------------------- TIME INJ.---------------- TEST TIME------------ INJECTION ------------------------- RESULT--------------- RESULT DATE
2/27/12----------------------------- RIPTROPIN-------------- 0830 ---------------- 1202-------------- IM ------------------------------- 30.2 ng/mL -------------------- 2/28/12
  #65  
Old 02-28-2012, 11:24 AM
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I will post the last two scanned lab results and vials with batch date/numbers tonight.
  #66  
Old 02-28-2012, 11:50 AM
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I'd say Rips are pretty good. Kind of blowing everything else away.
  #67  
Old 02-28-2012, 11:56 AM
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I would say yes, with a difference like that there will be a better result with the same dose.
  #68  
Old 02-28-2012, 12:25 PM
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I will post the last two scanned lab results and vials with batch date/numbers tonight.
great work madmoe....found a medlab lacation near me and am going to get some testing ......so is fasting required for natural gh testing but if taking supplemental gh no need to fast?

thanks snake
  #69  
Old 02-28-2012, 02:20 PM
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LABTEST DATE--------------------- HGH------------------- TIME INJ.---------------- TEST TIME------------ INJECTION ------------------------- RESULT--------------- RESULT DATE
2/27/12----------------------------- RIPTROPIN-------------- 0830 ---------------- 1202-------------- IM ------------------------------- 30.2 ng/mL -------------------- 2/28/12
Holy Shit! Now that's a surprise. These are all at 10ius, right? Except the pharm grade.
  #70  
Old 02-28-2012, 04:41 PM
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Great thread, man. This is great information and much appreciated.
  #71  
Old 02-28-2012, 04:54 PM
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Holy Shit! Now that's a surprise. These are all at 10ius, right? Except the pharm grade.
all 10 ius but Tev
  #72  
Old 02-28-2012, 04:57 PM
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Originally Posted by snakeskinz View Post
great work madmoe....found a medlab lacation near me and am going to get some testing ......so is fasting required for natural gh testing but if taking supplemental gh no need to fast?

thanks snake
no need to fast at all.
  #73  
Old 02-28-2012, 05:10 PM
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Look forward to hearing bout the elis..believe he is making a newer "supposed" better hgh soon??
  #74  
Old 02-28-2012, 05:28 PM
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Issue with Elis come down to no batch date on box or vial. That makes it nearly impossible to pinpoint the date of manufacture.
  #75  
Old 02-28-2012, 05:40 PM
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Default Labcorp Results Rips

Labcorp Results Rips
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File Type: jpg RIPS RESULTS.jpg (56.9 KB, 689 views)
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